Isolation and expression ofPax6andatonalhomologues in the American horseshoe crab,Limulus polyphemus
نویسندگان
چکیده
منابع مشابه
Sediment microbiomes associated with critical habitat of the Juvenile American Horseshoe Crab; Limulus polyphemus
Plumb Beach, Brooklyn, New York in USA is an important horseshoe crab breeding and nursery ground that has experienced substantial anthropogenic influence, including pollution, erosion and subsequent restoration. Since little is known about the relationship between sediment microbial communities and juvenile horseshoe crab survival, next generation sequencing was used to characterize and compar...
متن کاملBlood Collection from the American Horseshoe Crab, Limulus Polyphemus
The horseshoe crab has the best-characterized immune system of any long-lived invertebrate. The study of immunity in horseshoe crabs has been facilitated by the ease in collecting large volumes of blood and from the simplicity of the blood. Horseshoe crabs show only a single cell type in the general circulation, the granular amebocyte. The plasma has the salt content of sea water and only three...
متن کاملDevelopmental ecology of the American horseshoe crab Limulus polyphemus
During spawning events, horseshoe crab eggs are released from the female’s oviducts, and fertilized by one or more males. Eggs are shaped by the female into discrete clutches deposited in nests at depths of 10−20 cm on intertidal estuarine beaches. Distinguishing between fresh eggs and the early developmental stages is obfuscated by the large amount of dense, opaque yolk. The first unambiguous ...
متن کاملHemocyanin of the Horseshoe Crab, Limzdus polyphemus
The high molecular weight hemocyanin found in the hemolymph of the horseshoe crab, Limulus polyphemus, is composed of at least eight different kinds of subunits. Ion exchange chromatography at high pH in the presence of EDTA yields five major zones, hemocyanins I to V, three of which are electrophoretically heterogeneous. The subunits have similar molecular weights, 65,000 to 70,000, and their ...
متن کاملIsolation, purification and characterization of an iron-binding protein from the horseshoe crab (Limulus polyphemus).
The presence of an iron-binding protein in the haemolymph of the horseshoe crab, Limulus polyphemus, was detected by gel filtration of 59Fe-labelled haemolymph. Lysis of amoebocytes did not change the amount of iron-binding protein in haemolymph samples. The protein was purified to homogeneity by ion-exchange chromatography. The molecular mass of the purified protein was estimated to be 282,000...
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ژورنال
عنوان ژورنال: Developmental Dynamics
سال: 2008
ISSN: 1058-8388,1097-0177
DOI: 10.1002/dvdy.21634